Composite

Part:BBa_K5089007

Designed by: Camille Prefontaine   Group: iGEM24_UManitoba   (2024-09-29)


MGS0156-CsgA-LCI

This composite part consists of all components required for expressing and displaying MGS0156 and LCI on the cell surface of E.coli BL21(DE3). The characterization of the enzymatic activity of MGS0156 is available in the documentation for the base part BBa_K4949002. The characterization of LCI binding ability can be found in the documentation of the base part BBa_K3013000.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 457
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1059
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 159
    Illegal NgoMIV site found at 330
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 843


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Categories
Parameters
None